Journal: Molecular Medicine Reports
Article Title: Polygonatum sibiricum polysaccharide alleviates liver fibrosis through the TGF-β/Smad signaling pathway and reduces collagen
doi: 10.3892/mmr.2025.13599
Figure Lengend Snippet: PSP inhibits hepatic collagen production in liver fibrosis-induced rats. Detection of the liver fibrosis markers (A) HA, (B) LN, (C) PIIINP and (D) Col IV in serum samples was performed by ELISA. (E) Expression levels of Col I and Col III in the liver were detected by immunohistochemistry; scale bar, 20 µm. Semi-quantification of (F) Col I and (G) Col III. ELISA was used to detect the levels of (H) Col I and (I) Col III in liver tissue. (J) Levels of Hyp in the liver tissues were detected by ELISA. Data are presented as the mean ± standard deviation, n=6. ### P<0.001 vs. the control group; *P<0.05, **P<0.01 and ***P<0.001 vs. the model group. BJRG, Biejia ruangan; Col, collagen; H, high; HA, hyaluronic acid; Hyp, hydroxyproline; L, low; LN, laminin; PIIINP, procollagen III N-terminal peptide; PSP, Polygonatum sibiricum polysaccharide.
Article Snippet: Goat serum (cat. no. c-0005), MMP2 (cat. no. bs4605R), MMP9 (cat. no. bsm55544m), Col III (cat no. bs-0549R) and Col I (cat no. bs-7158R) antibodies were obtained from Bioss.
Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemistry, Standard Deviation, Control